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MEDICAL SCIENCE

Notes On Histological Techniques

300

by Dr. Arpan Haldar

ISBN Number : 978 - 978 - 88672 - 14 - 6


Authors Details

Author Name Image About Author
Dr. Arpan Haldar
I passed MD (Anatomy) from Utkal University in 2014. Then I worked as Tutor in Department of Anatomy, ESI Post Graduate Institute of Medical Science, Joka, Kolkata under Ministry of Labour and Employment, Govt. of India. I current work as Senior Resident, Department of Anatomy, All India Institute of Medical Sciences Bhubaneswar, PMSSY Division, Ministry of Health and Family Welfare, Govt. of India. I have experience in special staining or organs and bone soft aborted human foetuses of various weeks of gestation as part of my Thesis Work during MD-Anatomy in Utkal University. I have experience in Hands on Training on FISH, Karyotyping, DNA Extraction and Chromosome Banding, PCR, Microarray, Sanger Sequencing done in Department of Cytogenetics, CMC Vellore and Department of Medical Genetics, SGPGIMS Lucknow. I have experience in Hands on Training on TEM, SEM and IEM done in Department of Anatomy, AIIMS New Delhi. I have done CME and Workshops on Medical Education & Bioethics in MEU ESI-PGIMSR Joka Kolkata and AIIMS Bhubaneswar. I have undergone CME on Ethics in Clinical Research by ICMR. I have done Workshop on Research Methodology & Scientific Paper Writing in Department of Epidemiology, NIMHANS Bangalore. I have done CME on updates in Cross-Sectional Anatomy by Radiological Approach by Department of Anatomy, AIIMS Bhubaneswar I have done CME on Use of Ultrasonography in Teaching and Learning Anatomy in KGMC Lucknow. I have also done CME on Genesis and Role of Microteaching in Anatomy I have also done Hands on Workshops on Soft Embalming on Cadaveric Surgery in KGMC Lucknow. I have also done Hands on Workshops on Model Making in AIIMS Bhopal. I have special interest in Teaching and Research in Neuroanatomy, Embryology and Teratology, Genetics, Histology and Cell Biology. I also participate in the Cadaveric Workshops conducted in the Department of Anatomy, AIIMS Bhubaneswar in collaboration with Orthopaedics, PMR, ENT and Neurosurgery. I am doing Hands on Immunohistochemistry in AIIMS Bhubaneswar assisting in various Intramural and Extramural projects as Co-PI. I have done Hands on National Training on COMET Assay in Department of Anatomy, JIPMER Pondicherry. I have undergone DST Funded Short Term Training on Electron Microscopy in Department of Anatomy, AIIMS New Delhi. I have undergone ICMR Funded Short Term Training on Medical Genetics in Department of Medical Genetics, SGPGIMS Lucknow. I have also undergone Short term training on Biostatistics, Epidemiology & SPSS in Department of Biostatistics, CMC Vellore. I am regularly presenting Oral and Poster Papers at the National Conferences twice a year and regularly go to Short-term trainings and Hands on Workshops. I am also involved in teaching MBBS, Paramedicals and Nursing and routinely take practical and lectures, Dissection, Embalming duties. I am in Editorial Board Member in 10 Indexed International Journals and Reviewer in 3 International Indexed Journals. I am Life Member of Anatomical Society of India, Society of Clinical Anatomists of India, Electron Microscope Society of India, American Association of Clinical Anatomists, Indian Academy of Medical Genetics and Indian Academy of Neurosciences. I have also proposed a chapter on Embryological Implications of Argentaffin/Enterochromaffin cells in a book “Serotonin Odyssey” [Editor: Prof PK Debnath, Foreword by Prof MN Ghosh and Preface by Dr ADB Vaidya]. I have presented till date 15 Oral and Poster papers in the various National and State Conferences. I have published 28 articles in Indexed National and International Journals. I am involved in Ultrastructure of Aborted human foetuses by use of SEM and TEM for knowing the detailed architecture of cell organelles of foetuses of different weeks of gestation in collaboration with Institute of Physics, Bhubaneswar. I am involved in Genetic Analysis of MTHFR Gene in Anencephalic Fetuses with Spina Bifida by use of Sanger sequencing, Microarray, TP-PCR, QF-PCR in collaboration with Department of Medical Genetics, SGPGIMS Lucknow. I am involved in detection of DNA damage in various human foetuses of various weeks of gestation in hypertensive and diabetic mothers by COMET Assay (Single Cell Gel Electrophoresis) for estimation of DNA damage in collaboration with JIPMER Pondicherry. I am involved in detection of any genetic abnormalities in children with congenital malformations and childhood tumours for future targeted drug delivery system in utero by nanoparticles in collaboration with Department of Cytogenetics CMC Vellore. I am involved in Inter-departmental papers on “Distribution and Expression of neonatal IgG Fc receptor, FcRn in the Fetal Human Tissues and Placenta in collaboration with Dept. of Obs Gynae and Pathology, AIIMS Bhubaneswar. I was Invited Guest Speaker for Presentation in International Conferences of World Congress on Stem Cell and Biobanking 2018 on 3rd and 4th Sept 2018 in Tokyo, Japan and Global Experts Meeting on Frontiers in Cell & Stem Cell Research 2019 from April 18-20, 2019 in New York, USA. I was invited as Delegate in Annual Conference of Royal College of General Physicians from 4th-6th Oct 2018 in Glasgow, UK and in International Meeting of ANATOMIA CLINICA 24th to 26th June 2019 in Madrid (Spain). I am involved as Co-PI in an Extramural Project funded by Ministry of AYUSH, Govt of India on Expression of CD30 antigen in Human Foetal Tissues by Ber-H2 and an Intramural Project Institute Funded on Immunohistochemical analysis of axonal regeneration through peripheral nerve grafts containing Schwann cells expressing BDNF, CNTF or NT3 in patients after Peripheral nerve Injury. I am the Program Advisor for Royal College examination training program at Member of Royal College of Physicians College of PG Medicine, Texila American University in collaboration with University of Central Nicaragua. I was awarded Memento in IMS-BHU for oral paper presentation on Developmental Neuroanatomy of Cerebral and Cerebellar Cortex, Brain Stem and Spinal Cord in Human Fetuses In Various Gestational Weeks Of In Eastern Geographical Region of Odisha in International Conference of Neurosciences and Annual Conference of Indian Academy of Neurosciences on November 2018. I have attended International Conference of Asian Network of Research on Antidiabetic Plants: Prevention& Management of Diabetes-Prospects & Challenge in September 2012 and presented paper on Histogenesis& Cytogenesis of Islets of Langerhans in Fetal Pancreas with implications to Periampullary Carcinoma in the Head of Pancreas on Sept 2012. My special research interests are in Neuroanatomy & Neurobiology, Histology & Cell Biology, Embryology & Teratology, Genetics and Physical Anthropology, Molecular Cell Biology.

Book Description

Histological techniques are the crucial steps of Histology, Cell Biology and Histopathology as Molecular Cell Biology and Histopathology of Biopsied specimens are of utmost importance now a days. The chemical and techniques required for making the Histological stains is helpful for Postgraduates and Laboratory Technicians. The compositions, reagents, steps of H&E and other special stains are well illustrated in this book. The recent techniques of Immunohistochemistry and Electron Microscopy are also incorporated in this book. This book is written in view of increasing demand of a short notebook on Histological techniques for biological samples or tissues. The first edition highlights the histological techniques, constituents with a view of the steps of Immunohistochemistry, Scanning Electron Microscopy, Transmission Emission Microscopy and Immunoelectron Microscopy. The author is particularly indebted to Prof. Manisha R Gaikwad, Additional Professor and Head, Department of Anatomy, AIIMS Bhubaneswar for her valuable proof reading of the book. The author also acknowledges Prof. Dipti Basu, Ex- Principal and Professor, Department of Anatomy, Calcutta National Medical College. The author also noted the valuable comments from Prof. Soumya C Bhattacharya, Professor and Head, Department of Anatomy, ESI-Post Graduate Institute of Medical Sciences and Research, Joka, Kolkata. Lastly the author also acknowledges the Almighty, Dad, Mom, Wife and Daughter for supporting this work. The author is also indebted to all the Cadavers, students, colleagues, seniors and juniors for their inspiration. The author hopes that this book enriches the knowledge of techniques, constituents and methodology of preparations of histological stains, immunohistochemical and steps of electron microscopy.


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